(none)

[Venkat Pisupati]

In article <v01540b06ae2e793bea77@[130.236.200.20]>,
Sailesh.Surapureddi at mcb.liu.se (Sailesh Surapureddi) wrote:

> Hi  Everybody
> 
>         Here is the ice cold transformation protocol.. I am posting this
> for the benifit all transformers.
> 

snip
 
> Low! you have your tranformations with the higest effiencies you have seen
> so far. If you are using some DNA, make sure its clean. Ofcourse! for me,
> it worked with even the yeast shuttle vectors. They were crude preparations
> btw.
> 

what sort of efficiency  do you get wtih this protocol. (no of
transformants/ug of DNA)

-- 
Venkat Pisupati                                                        Lab  -44-01223-334131
Wellcome/CRC Institute                                           Fax -44-01223-334134
Dept. of Genetics                                                      E.mail. vnp at mole.bio.cam.ac.uk
University of Cambridge
UK CB2 1QR