freeze-squeeze (Re: geneclean: vortexing)

Eitan Rubin bcrubin at dapsas1.weizmann.ac.il
Wed Aug 7 10:56:12 EST 1996


You can ETOH precipitate the Freeze-squeezed DNA and get it in water, 
but than yields are lower than geneclean (I got 10% in 2 trials - which 
was fine with me).
  I rutinly reamplify minor PCR products freeze-squeezed in the shortest 
possible protocol: I add 0.2 ml ddw to the gel slice, freeze it in liq. 
nit. and centrifuge 5 min, and than use 2 microliter as template. I 
still have to try the same protocol without freezing...
  Eitan.



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