fjalali at credit.erin.utoronto.ca
Wed Aug 7 15:51:56 EST 1996
I am trying to isolate RNA from a cyanobacterium (Synechococcus PCC 7942)
and in many of the protocols I have been reviewing they say to use water
saturated phenol. I have a couple of questions regarding this. Firstly,
how come water saturated, and not buffer saturated. Secondly, and I hope
this doesn't sound too stupid, how do you saturate the phenol with water.
When do you know enough is enough.
Any help would be greatly appreciated.
Department of Botany
University of Toronto
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