MICROEXTRACTION.

Fraser Dr N J njf16154 at ggr.co.uk
Tue Aug 6 04:31:48 EST 1996


<@medicine-dmb.newcastle.edu.au> wrote:
>Hello out there,
>
>I have a very robust PCR for sex determination and would like to test it 
>on pre-implantation embryos.  Does anyone have a protocol for 
>microextraction of DNA,  thanks in advance.
>
>Dale..
>
>
Try boiling them in a small volume of water (10-20ul), centrifuge down any
debris and use the supernatant directly. Works for up to 1000 cells, many more
and the PCR tends to be inhibited by something. Have tried it on mouse
blastocysts before and it worked. Hope this helps - Neil.




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