RNA extraction

Fraser Dr N J njf16154 at ggr.co.uk
Thu Aug 8 08:17:12 EST 1996


Farid jalali <fjalali at credit.erin.utoronto.ca> wrote:
>Hello, 
>
>I am trying to isolate RNA from a cyanobacterium (Synechococcus PCC 7942)
>and in many of the protocols I have been reviewing they say to use water 
>saturated phenol. I have a couple of questions regarding this. Firstly, 
>how come water saturated, and not buffer saturated. Secondly, and I hope 
>this doesn't sound too stupid, how do you saturate the phenol with water. 
>When do you know enough is enough.
>

1) With water saturated phenol, due to the lower pH, DNA will partition into
the organic phase leaving RNA in the aqueous phase.

2) You know when enough is enough when you get two separate phases.

Neil.




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