about transformation of DNA into E.Coli

Trevor Bezdek trevor at leland.stanford.edu
Thu Aug 8 16:00:24 EST 1996


I tried Sailesh's method last night and got a number of colonies, probably
not as many as standard protocol but I'll have to do a side-by-side check
tonight since I was also trying new competent cells at the time.  I simply
added 2 microliters of DNA from my ligation to 50 microliters competent
cells, left them for 3 minute on ice and then plated half of the cells on
an LB-amp plate prewarmed at 37° for 15 minutes.  It worked...

trevor



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