Mung bean nuclease ?
fourie at crimson
Thu Aug 8 12:07:41 EST 1996
Could someone help me out with the reaction conditions for Mung bean
nuclease? I need to convert a NcoI digested site to blunt ends, but I
seem to be losing most of my plasmid...
Some info on reaction times and units of enzyme / ug of DNA would
be very helpful!
Thanks in advance...
Department of Biochemistry
University of Pretoria
fourie at scientia.up.ac.za
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