Mung bean nuclease ?

Fourie Joubert fourie at crimson
Thu Aug 8 12:07:41 EST 1996


Hi

Could someone help me out with the reaction conditions for Mung bean
nuclease? I need to convert a NcoI digested site to blunt ends, but I
seem to be losing most of my plasmid...

Some info on reaction times and units of enzyme / ug of DNA would
be very helpful!

Thanks in advance...

--------------
Fourie Joubert
Department of Biochemistry
University of Pretoria
fourie at scientia.up.ac.za
http://suntiger.ee.up.ac.za/biodocs/home.html
+27-12-420-2011



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