Mung bean nuclease ?

Fourie Joubert fourie at crimson
Thu Aug 8 12:07:41 EST 1996


Could someone help me out with the reaction conditions for Mung bean
nuclease? I need to convert a NcoI digested site to blunt ends, but I
seem to be losing most of my plasmid...

Some info on reaction times and units of enzyme / ug of DNA would
be very helpful!

Thanks in advance...

Fourie Joubert
Department of Biochemistry
University of Pretoria
fourie at

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