Matthew P. Frosch, M.D., Ph.D. frosch at dsg.harvard.edu
Tue Aug 6 06:30:39 EST 1996

Would you be willing to share your PCR protocol?  I have tried one
protocol from the literature (Aasen & Medrano, "Amplification of the ZFY
and ZFX genes for sex identification in humans, cattle, sheep and
goats",Biotechnology, 8:1279 1990) but it does not appear to work in my
strain of mouse.

For embryo DNA preparation, you might look at Kunieda, et al. "Sexing of
mouse preimplanation embryos by detection of Y chromosome-specific
sequences using PCR", Biol. Reproduction, 46:692, 1992.

M. P. Frosch, MD, PhD
Brigham and Women's Hospital

frosch at dsg.harvard.edu

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