Sliver staining DNA

Paul N Hengen pnh at ncifcrf.gov
Thu Aug 8 11:40:35 EST 1996


Josh Udall (judall at uidaho.edu) wrote:

> I want to detect relatively small length polymorphisms amplified by
> microsatellite specific primers.  EtBr leaves too much back ground and
> isn't sensitive enough, radioisotopes are good but I want to try some
> alternatives.  
> 
> Does anyone have on file a protocol for sliver stain nucleic acids in
> polyacrylamide gels?  Possibly a few references would be nice.  I've
> seen a few 'staining kits' but they don't let on how its really done.

The silver stain will probably cause alot of background as well. You
could save yourself much fooling around if you use SYBR Green I, but
prepare to pay a little more money.

--
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