Sliver staining DNA

Josh Udall judall at uidaho.edu
Thu Aug 8 10:45:05 EST 1996


I want to detect relatively small length polymorphisms amplified by
microsatellite specific primers.  EtBr leaves too much back ground and
isn't sensitive enough, radioisotopes are good but I want to try some
alternatives.  

Does anyone have on file a protocol for sliver stain nucleic acids in
polyacrylamide gels?  Possibly a few references would be nice.  I've
seen a few 'staining kits' but they don't let on how its really done.

thanks

Josh Udall
University of Idaho



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