about transformation of DNA into E.Coli

Sailesh Surapureddi Sailesh.Surapureddi at mcb.liu.se
Fri Aug 9 05:10:34 EST 1996




Hi Trevor

        Glad that you were partially successful. But to improve your
tranformations.

1. Pre warm your plates for longer times. 15min, isnt good enough.. try
atleast 60min.. the plates should be really warm and dry.

2. Initally incubate your DNA with Cells for 5min till you get  the hang
out of your method.

3. Clean your ligation mixes, since the ligation buffers usually kill your
cells.

Good luck with more efficiencies.

Sailesh.

Dr.Sailesh Surapureddi
Plan 12; Inst. for Cellbiologi
Halsouniversitetet
S 581 85, Linkoping
Sweden.

Tel: +46-13-223917 (W) +46-13-138839 (R)
Fax: +46-13-224149
E.mail: Saisumcb at hulio.liu.se
        Saisu at mcb.liu.se





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