about transformation of DNA into E.Coli
Sailesh.Surapureddi at mcb.liu.se
Fri Aug 9 05:10:34 EST 1996
Glad that you were partially successful. But to improve your
1. Pre warm your plates for longer times. 15min, isnt good enough.. try
atleast 60min.. the plates should be really warm and dry.
2. Initally incubate your DNA with Cells for 5min till you get the hang
out of your method.
3. Clean your ligation mixes, since the ligation buffers usually kill your
Good luck with more efficiencies.
Plan 12; Inst. for Cellbiologi
S 581 85, Linkoping
Tel: +46-13-223917 (W) +46-13-138839 (R)
E.mail: Saisumcb at hulio.liu.se
Saisu at mcb.liu.se
More information about the Methods