OD at 320nm

Jose E. Mejia jose.mejia at well.ox.ac.uk
Fri Aug 9 15:27:46 EST 1996

In article <elauzier-0808961336310001 at mac114.peps.ulaval.ca>,
elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:

> It doesn't necessary mean that whatever absorb at 320 will absorb too at
> 260.  So by substracting the 320 value from the 260, am I not
> underestimating my RNA levels in my sample ? ( that is in the case the
> background is less at 260 or even nil because I have absolutly no proof
> that it absorb the same at 320 and 260 ! )...

I agree, but I think that OD at 320 nm measures light scattering by
insoluble material, which is different from light absorption at specific

J.E. Mejia

Wellcome Trust Centre for Human Genetics
Windmill Road, Oxford OX3 7BN, UK
Tel: +44 1865 740021
Fax: +44 1865 742186
Email: jose.mejia at well.ox.ac.uk

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