OD at 320nm

Jose E. Mejia jose.mejia at well.ox.ac.uk
Fri Aug 9 15:27:46 EST 1996


In article <elauzier-0808961336310001 at mac114.peps.ulaval.ca>,
elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:

> It doesn't necessary mean that whatever absorb at 320 will absorb too at
> 260.  So by substracting the 320 value from the 260, am I not
> underestimating my RNA levels in my sample ? ( that is in the case the
> background is less at 260 or even nil because I have absolutly no proof
> that it absorb the same at 320 and 260 ! )...

I agree, but I think that OD at 320 nm measures light scattering by
insoluble material, which is different from light absorption at specific
wavelengths.

J.E. Mejia

-- 
Wellcome Trust Centre for Human Genetics
Windmill Road, Oxford OX3 7BN, UK
Tel: +44 1865 740021
Fax: +44 1865 742186
Email: jose.mejia at well.ox.ac.uk



More information about the Methods mailing list