Asparagine synthetase selection

Masamichi Oshima masa at
Fri Aug 9 14:17:36 EST 1996

Dear b.m.m-r netters,

	Has anyone of you used the asparagine synthetase gene
as a dominant selectable marker?  It's described in the
famous Molecular Cloning book.  However, I don't see many
articles in which this selection marker is used.  Would
anyone know pros and cons of this selectable marker?
Would anyone be kind enough to e-mail me a good protocol
for Asp. synthetase selection?

Masamichi Oshima, M.D., Ph.D.
Department of Bacteriology
Faculty of Medicine
University of Tokyo
7-3-1 Hongo, Bunkyo-ku
Tokyo 113, Japan
E-mail: masa at

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