cDNA visualization on agarose, HELP!!!
gerardo at N-JCENTER.COM
Fri Aug 9 14:32:08 EST 1996
Could someone, please help with this puzzle?
I am doing RT-PCR with an arbovirus. As a routine, I check my positive
control and positive samples by spotting 1 microL on nylon membranes and
hybridize with oligonucleotide probe and detect them with a colorimetric
method without a problem. However, when I run aliquot of the samples on
agarose (3%), TAE, and EtBr stain, I don't see any bands!, except for
the molecular marker (0.1- 10 Kb). I should have amplified enough cDNA
to visualize it in the gel.
Any suggestion? I will appreciate it.
Palm Coast, Florida
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