chemical mutagenesis

Vlad Svetlov vvsvetlov at utmem1.utmem.edu
Sat Aug 10 04:05:21 EST 1996


In article <mt.6.000EC04E at dna.bio.warwick.ac.uk>, mt at dna.bio.warwick.ac.uk
(Mark Slack) wrote:

> I am currently trying to make a series of mutants by chemical
mutagenesis in a 
> 300bp DNA fragment and I am aiming for single hit.  


Pardon my curiosity, but why you using chemical mutagenesis to obtain
single mutations in such a short fragment of DNA? If your nitrite does not
work why not try something more robust like mutagenic PCR?

Regards,
V. S.



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