renaturing proteins

jgluz at STUD.MED.CORNELL.EDU jgluz at STUD.MED.CORNELL.EDU
Sat Aug 10 14:53:12 EST 1996


I have a few proteins expressed in E. coli that form inclusion bodies.  
I tried resuspending the stuff in 5M guanidine/buffer and slowly 
dialyzing out the guanidine.  No luck.  What concentration should my 
protein be at for this procedure?  Any suggestions on renaturing? Please 
send them along.  Thanks--luz






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