Help with nuclease S1 protocol

Dinakar desai at YELLOW.MED.YOKOHAMA-CU.AC.JP
Mon Aug 12 04:35:28 EST 1996


Hello Everyone:

I am looking for a good working protocol of Nuclease S1 assay to 
determine the 5'end of mRNA.  I have only double stranded DNA. Can 
anyone help me with good protocol that works with double stranded DNA.

Thanks

With best regards

Dinakar 
======================================
Dr. Dinakar Desai, Ph.D.
Department of Molecular Biology
Yokohama City University
School of Medicine
3-9, Fuku-ura, Kanazawa-ku
Yokohama 236, Japan
Ph: 81-045-787-2597, 2598
Fax: 18-045-785-4140
email: desai at med.yokohama-cu.ac.jp
or desaid at marshall.edu
http://webpages.marshall.edu/~desaid/
======================================



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