Indicators for electroporation eficiency
darjania at CRIBA.EDU.AR
Tue Aug 13 15:13:12 EST 1996
Deat group members
I am going to electroporate guard cell protoplsats, isolated from Vicia
faba, to load cAMP and ATP, which are not memebrane permeable. The use of
cAMP/AM is not too desirable because of AM toxic group.
To adjust electroporation protocol I need some colorful, non-permeable
indicator with MW more or less similar to cAMP or ATP.
Regarding to this, could anyone gave me some information about such
indicators? Evans blue ... is not suitable in this case.
Thanks for your assistance.
e-mail: darjania at criba.edu.ar
More information about the Methods