MluI cuts like it wants

Volker Noelle a2239221 at SMAIL1.RRZ.UNI-KOELN.DE
Tue Aug 13 12:04:54 EST 1996


Hi,

I have a problem with the restriction enzyme MluI:

Vector with two MluI sites: MluI cuts.
Vector with Insert (total three MluI sites): MluI doesn't cut!!
Insert isolated from vector (one MluI site): MluI cuts!

What's that???

MluI is not affected by dam/dcm methylases. All DNA is made in E.coli
DH5alpha.

Any idea?

Thanks Volker



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