transcription termination

ae at ae at
Tue Aug 13 14:38:46 EST 1996

In article <320FBFF3.4EDD at> Andrew Brooks
 <Andrew.Brooks at> writes:

>I wish to produce RNA in the cytoplasm of mammalian cells without a 
>poly-A tail. Transcription should not be a problem, but my big problem is 
>that I want to terminate transcription exactly after my insert. 

The best way to be sure of termination at a prcise point in a eukaryotic 
system is to include a ribozyme such as that from hepatitis delta in the 
transcribed portion of the plasmid.  This can be placed to cleave the RNA at a 
chosen point.

Andrew Easton

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