Invitrogen's pZERO & E.coli with ccdA

Bret Sellman bsellman at
Tue Aug 13 10:32:11 EST 1996

I have been using the pZERO vector from invitrogen with great success. 
The only problem is that the vector is not cheap to buy and I cannot
propagate the plasmid without first cutting it and ligating in DNA. 
Does anyone know how to propagate this vector?  Are there any E. coli
strains available which overexpress ccdA. Thanks.

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