Permenant mounting of AP-NBT/BCIP slides

Wes Garrett wgarrett at ggpl.arsusda.gov
Tue Aug 13 17:02:52 EST 1996


When using non-radioactive ISH with alkaline phosphatase and NBT/BCIP
chromogen, how do you permenantly mount the slides.  This chromogen is
soluable in solvents, and I have had no luck with either alcohol
dehydration, or clearing in xylene.  I have been told that this works,
but I either lose signal, or develop crystals where the precipitate
forms.

thanks

Wes
wgarrett at ggpl.arsusda.gov




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