In situ hybridization on tissue sections

D. P. Wijesinghe DPWCC at CUNYVM.CUNY.EDU
Tue Aug 13 20:36:14 EST 1996


I am planning to do in situ hybridization on Drosophila larval sections.
If anyone has experience with this I would appreciate information on
the type of (histological) sections for this: paraffin, frozen or plastic.
 
 
In addition, I would appreciate any information on labelling of the probe.
I have used DIG-labelled probes for whole mount in situ hybridization -
is this suitable for sections?
 
Many thanks in advance.
 
Harshinee Wijesinghe
New York, NY
dpwcc at cunyvm.cuny.edu



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