Tricks for pipetting detergents??

brett brett at BORCIM.WUSTL.EDU
Wed Aug 14 13:14:14 EST 1996


>You could always weigh it. I make up a 10% solution this way which suits
>my needs.
>
>
>
>Glenn
>
>In article <4uqsu7$pb7 at apakabar.cc.columbia.edu>,
>pw at cubsps.bio.columbia.edu (Paul Wakenight) wrote:
>
>> I'm sure everyone has tried to pipette NP40, etc.  What a mess.
>> Outside of using a graduated cylinder, is there anyway to do this 
>> accurately?
>> 
>> thanks
>> 
>> Paul Wakenight
>> pw at cubsps.bio.columbia.edu

I agree. I make up a 10%(v/v) soln in 50ml graduated Falcon tubes (ca.5ml
detergent, vol. to 30ml. Vortex into soln., allow foam to settle, top up to
50ml.


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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