Sliver staining DNA
dkim at nmsu.edu
Sat Aug 10 11:10:59 EST 1996
In article <320A0B81.30D0 at uidaho.edu> judall at uidaho.edu writes:
>I want to detect relatively small length polymorphisms amplified by
>microsatellite specific primers. EtBr leaves too much back ground and
>isn't sensitive enough, radioisotopes are good but I want to try some
>Does anyone have on file a protocol for sliver stain nucleic acids in
>polyacrylamide gels? Possibly a few references would be nice. I've
>seen a few 'staining kits' but they don't let on how its really done.
>University of Idaho
Check out Bassam and Caetno-Annoles paper in ANalytical Biochemistry
(late 1980's, I think). They have a DNA silver stain method that is now
used by Promega in their Silver Sequence kit.
If you cannot do a search, you could ask Promega: They cite this paper
in their advertising/promotional stuff on the Silver Sequence.
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