Purification of VLP«s
unckf000 at goofy.zdv.uni-mainz.de
Wed Aug 14 11:11:50 EST 1996
Hi, I´m a graduate student and I´ve got some problems with the
purification of virus like particles. After the expression of the
relevant viral genes in mammalian cell culture, I subject the cell
lysate to two centrifugation steps (density and sedimentation
centrifugation). With EM imaging we can observe in some fraction an
impurity, which cause a slight agglomeration. Did anyone observe a
similar phenomenon and how did you solve this problem?
It would be kindly to contact me!
More information about the Methods