Purification of VLP«s

Felix Unckell unckf000 at goofy.zdv.uni-mainz.de
Wed Aug 14 11:11:50 EST 1996

Hi, I´m a graduate student and I´ve got some problems with the 
purification of virus like particles. After the expression of the 
relevant viral genes in mammalian cell culture, I subject the cell 
lysate to two centrifugation steps (density and sedimentation 
centrifugation). With EM imaging we can observe in some fraction an 
impurity, which cause a slight agglomeration. Did anyone observe a 
similar phenomenon and how did you solve this problem?
It would be kindly to contact me! 

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