Volker Noelle wrote: > [snip] > Vector with Insert (total three MluI sites): MluI doesn't cut!! > [snip] Just a guess, but either you prepped the wrong thing for V+I, or it's a dirty prep. --- --- --- Richard J. Dudley Neurobiology Research Center Dep't of Physiology and Biophysics University of Alabama School of Medicine http://www.nrc.uab.edu