Mung bean nuclease ?

Karah Lanier lanier at niehs.nih.gov
Wed Aug 14 12:14:00 EST 1996


It is good idea to titer the amount of mung Bean nuclease one uses in 
the reaction since it has a tendancy to be overzealous in its activity 
when excess enzyme is around. I used it to make a blunt end and it 
resulted in clones with  a variety of different termini when 
sequenced. 

I just transformed with ligations from several rxns with diffrent 
amounts of nuclease and sequenced several clones, many were diffrent 
but i got the one i wanted!

Nigel Walker Ph.D
walker3 at niehs.nih.gov



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