Mung bean nuclease ?
lanier at niehs.nih.gov
Wed Aug 14 12:14:00 EST 1996
It is good idea to titer the amount of mung Bean nuclease one uses in
the reaction since it has a tendancy to be overzealous in its activity
when excess enzyme is around. I used it to make a blunt end and it
resulted in clones with a variety of different termini when
I just transformed with ligations from several rxns with diffrent
amounts of nuclease and sequenced several clones, many were diffrent
but i got the one i wanted!
Nigel Walker Ph.D
walker3 at niehs.nih.gov
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