Cosmids

[Peter J. Myler]

We too have had very good results with SuperCos.  It also seems to make
very good DNA for digests and sequencing.

Regards

Peter 
-- 
====================================================================
Peter J. Myler
Seattle Biomedical Research Institute
4 Nickerson Street
Seattle, WA  98109-1651
phone: (206) 284-8846x332
FAX: (206) 284-0313
e-mail: MYLERPJ at U.WASHINGTON.EDU
====================================================================





J. Pat Martinez <martinez at puccini.CRL.umn.edu> wrote in article
<martinez-1408961705420001 at mac20.agoff.umn.edu>...
> I've used Supercos from Stratagene with good results.  One advantage with
> this cosmid is that you don't have to size select your partially digested
> DNA.  The design of the vector (2 cos sites) allows the packaging
reaction
> to select the appropriate sized recombinant.  I've only used this cosmid
> vector so I don't know how it compares to other cosmid vectors.
> 
> Pat M.
> 
> 
>  <Pine.A32.3.93.960813211140.34344A-100000 at kitts.u.arizona.edu>, Stephen
J
> Billington <sbilling at U.Arizona.EDU> wrote:
> 
> > Hi Mol. Biol. Netters
> > 
> > I am looking to create genomic libraries from 2 or 3 microorganisms. I
> > have in the past used lambda derivatives with great success, but my new
> > boss is dead keen to use cosmids. His main point is that we can use the
> > cosmid directly to complement E. coli mutants. Anyway, not wanting to
> > argue with the boss, does anyone out there have any favorite cosmids?
My
> > major problem with thenm is the hassle with subcloning from such large
> > inserts and my few encounters with them in the past have been riddled
with
> > instability problems. If anyone can help me with this I'd be
appreciative,
> > the boss wants to use pLFA, but surely there is something a bit more up
to
> > date and easier to use than that.
> > 
> > Catch yu
> > 
> > SJB
> > 
> > ------------------------------------------------------------
> > Stephen Billington Ph.D.
> > Department of Veterinary Science
> > University of Arizona
> > sbilling at U.Arizona.edu 
> > billing at vetsci.microvet.arizona.edu
> 
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