Fiske and Subbarow reagent . . .
Dr E. Buxbaum
EB15 at le.ac.uk
Thu Aug 15 05:23:08 EST 1996
For the ANSA reagent dissolve 37.5 g Sodium metabisulfite, 0.625 g ANSA
and 2.5 g Sodium sulfite in 250 ml Aq. bidest. by warming and filter
while warm. Keep refrigerated in a brown bottle.
You also need a solution of 2.5% Ammonium molybdate in 2.5M sulfuric
Mix 4.5 ml Sample (up to 10 micro M phosphate) and 0.3 ml Molybdate, then
add 0.2 ml ANSA reagent and incubate 10' at RT. Read at 620 nm.
The publication is in JBC 66 (1925) 375-400
More information about the Methods