HELP!!! Lambda prep...
aisoai at yk.rim.or.jp
Wed Aug 14 21:56:58 EST 1996
In article <4udga1$qrj at knot.queensu.ca>, Allison <3abm4 at qlink.queensu.ca>
>I'm doing the "Standard method for purification of bacteriophage
>lambda" (Sambrook et al. pp 2.73-2.76) and at the end of step 11, the
>CsCl step gradient, I'm getting a blue band _above_ a white band, the
>opposite of what the protocol says should result. Also, the bands seem
>too far up in the tube. Does anybody know what is happening? Perhaps
>more importantly, does anybody recommend a better (more recent?)
>protocol? This one seems long and ripe for catastrophic errors on my
The standard method for isolation of phage DNA is laborious and
time-consuming. I had used Lambda Kit (QIAGEN Inc,) and confirmed it
works very well. Using this kit, you can omitt the step of CsCl-gradient
ultra-centrifugation and obtain good quality and quantity ofphage DNA.
I hope my suggestion is useful for you!
Atsushi Isoai, Ph.D.
Internet / aisoai at agc.co.jp (office) / aisoai at yk.rim.or.jp (home)
NIFTY / GBH02410
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