No banding in CsCl Spin,Why?

Jeremy Marcus marcusj at helix.mgh.harvard.edu
Thu Aug 15 11:41:58 EST 1996


In article <96227.154234ZXZ4 at psuvm.psu.edu>, Zhiyong Zhao
<ZXZ4 at psuvm.psu.edu> wrote:

: Yes, precipitate your DNA after Sol. 3. But do not precipitate it at -20C, you
: will have troubles with salt after spin. 30 mins at RT is usually enough.

...or, you could just add the isopropanol, and spin immediately.  Forget
the 30 minutes! This gives great results, although I wouldn't deny that
waiting 30' may enhance your yield.  

Jeremy



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