RPA, protect bigger band than probe

[Ned Mantei]

In article <4uv9gi$b7i at prometheus.acsu.buffalo.edu>,
xinguo at acsu.buffalo.edu (Xin Guo) wrote:
> Strangely, I saw a strong band (about 250 bp) protected.


Could this be double-stranded material? Perhaps the samples weren't
denatured, or renatured as they ran into the gel.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046