puromycin selection in e. coli and lymphoblastoid cell lines (LCLs)
aiyar at ebv.oncology.wisc.edu
Thu Aug 15 19:10:59 EST 1996
If you have used puromycin as a selectable marker in any suspension
cell lines, particularly lymphoblastoid cell lines or Burkitt's
lymphoma lines, I would much appreciate receiving your conditions as
a starting point for my titrations.
I have used puromycin in the 1 - 5 microgram/ml range for a few
murine and avian fibroblast-like lines.
I am also interested in hearing from anyone who has used puromycin
selection in E. coli.
Ashok Aiyar, Ph.D.
Department of Oncology email: aiyar at ebv.oncology.wisc.edu
University of Wisconsin-Madison tel: (608) 262-6697
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