puromycin selection in e. coli and lymphoblastoid cell lines (LCLs)

[Ashok Aiyar]

If you have used puromycin as a selectable marker in any suspension
cell lines, particularly lymphoblastoid cell lines or Burkitt's 
lymphoma lines, I would much appreciate receiving your conditions as 
a starting point for my titrations.

I have used puromycin in the 1 - 5 microgram/ml range for a few
murine and avian fibroblast-like lines.

I am also interested in hearing from anyone who has used puromycin
selection in E. coli.

Ashok
-- 
Ashok Aiyar, Ph.D.
Department of Oncology            email: aiyar at ebv.oncology.wisc.edu
University of Wisconsin-Madison                  tel: (608) 262-6697