PCR - SSCP loading mixture/denaturant??

alex parker aparker at maine.maine.edu
Fri Aug 16 07:34:15 EST 1996


Howdy.  I'm trying to get set up to do "cold SSCP" a la Hongyo et al (NAR
21:3637-3642), and am wondering if there is an equally good
alternative to their use of methylmercury hydroxide as a chemical
denaturant in the gel loading buffer.  They state that NaOH is inferior
in performance in their hands; anybody else's experiences would be
very much appreciated, as I'd rather not deal with this expensive
and doubtless toxic compound if it can be avoided.


Thanks in advance,

Alex Parker
aparker at maine.maine.edu



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