Bind-Silane and sequencing

Jim Hutchins hutchins at netdoor.com
Fri Aug 16 09:04:16 EST 1996


Frank Murphy (f-murphy at uiuc.edu) wrote:
: Seriously.  I don't quite understand the idea of making the gel stick to
: one plate anyway.   It always seems to go to one plate or the other when
: both plates are silanized
: 

That's because you don't know of the Bio-Rad sequencing apparatus, which
is awfully nice but uses a 'regular' glass plate for one side and
something called an Integral Plate-Chamber for the other side.  If your 
gel sticks to the glass plate, fine.  If it sticks to the IPC, you're
screwed: the IPC cannot be put into gel fix and even if you could, it
would take gallons of the stuff.

-- 
Jim Hutchins    hutchins at netdoor.com   hutchins at umsmed.edu
http://fiona.umsmed.edu/~hutchins/
Annie 1/3 NA, 2/3 CD, HIC *** Kodiak SC (Snuggle Certified)
"Morde diem" ****** Annie's motto ****** "Bite the Day"



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