Some polymerase better for PCR?

Vladimir Svetlov vvsvetlov at utmem1.utmem.edu
Thu Aug 15 19:55:06 EST 1996


In article <321361DA.28F4 at whale.st.usm.edu>, Shiao Wang
<swang at whale.st.usm.edu> wrote:

 
> Does anyone know of literature or have direct experience on which 
> thermal stable polymerase is more likely to work under "difficult" 
> conditions? 

We had several problems with amplifications using Taq that seem to be due
to contaminants in oligos and (once) template preps (confirmed by
inhibition of unrelated reactions). Switch to Pwo increased the yield
appr. 50-fold. It's also has greater fidelity than Taq.
Regards,
VS



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