RNA PURIFICATION

Stephen Smith sgsmith at tcd.ie
Sat Aug 17 08:55:11 EST 1996


Hello AJ, 
With regard to removing contaminating DNA from RNA samples, have you 
tried selective precipitation of the RNA?

Add an equal vol. of 5 M Ammonium Acetate to sample, incubate on ice 
20 min, pellet RNA by centrifugation  @ 10,000 x g @ 4 degrees for 10 
min. Wash pellet with 70% EtOH, dry briefly and resuspend in RNase 
free water (you may wish to add RNase inhibitor such as RNasin 
(Promega)).

All the best,
Stephen.

ps Your DNase might not be working due to lack of (or chelation of) 
Mg2+/Ca2+ in reaction mix.



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