sgsmith at tcd.ie
Sat Aug 17 08:55:11 EST 1996
With regard to removing contaminating DNA from RNA samples, have you
tried selective precipitation of the RNA?
Add an equal vol. of 5 M Ammonium Acetate to sample, incubate on ice
20 min, pellet RNA by centrifugation @ 10,000 x g @ 4 degrees for 10
min. Wash pellet with 70% EtOH, dry briefly and resuspend in RNase
free water (you may wish to add RNase inhibitor such as RNasin
All the best,
ps Your DNase might not be working due to lack of (or chelation of)
Mg2+/Ca2+ in reaction mix.
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