HELP!!! Fungal contamination of IMPORTANT transfectants!

Glenn Jenkins gjenkins at facstaff.wisc.edu
Sun Aug 18 14:23:15 EST 1996


There are several things you can do. Besides nystatin (fungazone), you
could do a limiting dilution of your cells in a 96 well plate. This will
also give you a colonal line if you haven't done this already. If you use
the fungazone method, you might want to test it with a split of your cell
line since it appears to have some toxicity associated with it in some
cells. 



Glenn  



In article <32168E3C.2E42 at rex.re.uokhsc.edu>, kprillim at rex.re.uokhsc.edu wrote:

> To anyone who can help me:
> 
> The past four months of my work is hanging in the balance as I write
> this, so ANY responses will be met with great enthusiasm!
> 
> It appears that some transfectants which I have been slaving away to
> make have become fungally contaminated during their lengthy incubation
> under G418 selection in my CO2 incubator.  Is there ANYTHING I can do to
> save these cells ("clean them up"), or are my next 3-4 months to be
> spent remaking all of my transfectants?
> 
> THANK YOU FOR YOUR HELP!
> 
> Kiley R. Prilliman
> University of Oklahoma Health Sciences Center
> Oklahoma City, OK



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