al antibodies against soybean BBI needed
wencenlaus at aol.com
Sat Aug 17 09:42:37 EST 1996
The following refers to a monoclonal/hybridoma provided by ATCC, clone
C238, product #HB-9657. (From MSRS Catalog. see www.ANTIBODIES-PROBER.com
for further info. on catalog)
Current medium for propagation: RPMI 1640 with 2 mM L-glutamine, 1 mM
sodium pyruvate and 10 mM HEPES, 85%; horse serum, 15%. Additional
Information: The C238 hybridoma produces mouse IgG2a lambda monoclonal
antibody that reacts with Bowman-Birk inhibitor. References: U.S. Pat.
5,053,327; J. Agric. Food Chem. 37: 1192-1196, 1989; ibid., 39: 327-335,
1991; Crop Sci. 32: 1502-1505, 1992; Brandon, D.L. et al. ELISA analysis
of soybean trypsin inhibitors in processed foods. In: Nutritional and
toxicological impact of food processing. M. Friedman, ed. New York: Plenum
Press; 1991:pp. 321-337; Brandon, D.L. et al. Antigenicity of soybean
protease inhibitors. In: Protease inhibitors as cancer chemopreventive
agents. W. Troll and A.R. Kennedy, eds. New York: Plenum Press; 1993:pp.
107-129. Depositor: D.L. Brandon, ARS Western Regional Research Center,
Albany, CA. Note: This material is cited in a U.S. and/or other Patent and
may not be used to infringe the patent claims.
More information about the Methods