some polymerase better than Tag?

Jin-seon Im jim at U.ARIZONA.EDU
Mon Aug 19 00:53:03 EST 1996


> Does anyone know of literature or have direct experience on which 
> thermal stable polymerase is more likely to work under "difficult" 
> conditions? 

We had several problems with amplifications using Taq that seem to be due
to contaminants in oligos and (once) template preps (confirmed by
inhibition of unrelated reactions). Switch to Pwo increased the yield
appr. 50-fold. It's also has greater fidelity than Taq.
Regards,
VS

What is Pwo ??

thanks in advance

jsim





More information about the Methods mailing list