Tricks for pipetting detergents??

H. Yim hyim at u.washington.edu
Mon Aug 19 12:19:05 EST 1996


For pipeting detergents or any viscous solution, I usually use an
appropriately sized syringe.  They are graduated, sterile and fast.

Harry Yim
Mailstop:CH-32
Dept of Infectious Diseases
Children's Hospital and Medical Center
Seattle WA 98105

hyim at chmc.org
hyim at u.washington.edu
(206) 363-1124

On 14 Aug 1996, brett wrote:

> >You could always weigh it. I make up a 10% solution this way which suits
> >my needs.
> >
> >
> >
> >Glenn
> >
> >In article <4uqsu7$pb7 at apakabar.cc.columbia.edu>,
> >pw at cubsps.bio.columbia.edu (Paul Wakenight) wrote:
> >
> >> I'm sure everyone has tried to pipette NP40, etc.  What a mess.
> >> Outside of using a graduated cylinder, is there anyway to do this
> >> accurately?
> >>
> >> thanks
> >>
> >> Paul Wakenight
> >> pw at cubsps.bio.columbia.edu
>
> I agree. I make up a 10%(v/v) soln in 50ml graduated Falcon tubes (ca.5ml
> detergent, vol. to 30ml. Vortex into soln., allow foam to settle, top up to
> 50ml.
>
>
> Brett Lindenbach
>
> Program in Immunology
> Washington University - St Louis
> brett at borcim.wustl.edu
>
> "I own my own pet virus. I get to pet and name her." - Cobain
>
>
>




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