Detecting HISx6

Michael W. Thompson mthom0 at
Mon Aug 19 11:54:44 EST 1996

     Again, I'll post this reference.  You might think about giving this one a try:

McMahan SA and Burgess RR.  Single-setp synthesis and characterization of biotinylated 
nitriloacetic acid, a unique reagent for the detection of histidine-tagged proteins 
immobilized on nitrocellulose. (1996)  Anal Biochem 236, 101-106.

More information about the Methods mailing list