anti-heparin and EDTA PCR tricks

jbw.hammond at ukonline.co.uk jbw.hammond at ukonline.co.uk
Tue Aug 20 11:23:22 EST 1996


Hi Dan
We treat blood before PCR as follows:
centrifuge 200ul for 3min.
Resuspend supernatant in 200ul of sdw. Freeze at -20 for 20min, thaw. Boil
in water bath for 5min. Centrifuge for 3min and use 20ul of supernatant
for PCR. The boiling destroys a Taq inhibitor in blood. This works for b-globin
PCR in our student practicals.

John Hammond
Faculty of Science, Nescot
UK



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