Northern Normalization

Heinz-Juergen Schaefers mfm434 at
Wed Aug 21 08:45:17 EST 1996

Susan Patrick wrote:
> I was hoping to use an internal standard, a so-called constituitively
> expressed gene, to normalize my northerns.  I have read some about how
> much these genes may vary in some cells, during differentiation, etc.
> Any suggestions on how I should go about insuring loading consistent
> amounts of RNA?  (I am working with differentiating F9 (murine) cells.)

Hi Susan,

the best internal standard for RNA loading is 18s or 28s rRNA.

Dipl. Biol. Heinz-Juergen Schaefers
University of Erlangen, Med. IV, Nephrology Research Lab
E-mail: university: mfm434 at
           private: h-j.schaefers at

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