Northern Normalization

Susan Patrick patrics at okstate.edu
Tue Aug 20 22:43:10 EST 1996


I was hoping to use an internal standard, a so-called constituitively 
expressed gene, to normalize my northerns.  I have read some about how 
much these genes may vary in some cells, during differentiation, etc.  
Any suggestions on how I should go about insuring loading consistent 
amounts of RNA?  (I am working with differentiating F9 (murine) cells.)

Thanks for any advice!

Susan Patrick
email:  patrics at okstate.edu





More information about the Methods mailing list