anti-heparin and EDTA PCR tricks
Dan.Andrews at anu.edu.au
Tue Aug 20 02:11:22 EST 1996
Hi to all in ether-land,
I have a couple of DNA samples that I have extracted from either EDTA-
or heparinized blood samples and unsuprisingly they are just about
impossible to amplify using PCR. I have tried using microcons and
ethanol precipitating the DNA to remove the heparin and EDTA, but to no
avail (I can amplify it just, but not always). I have heard that you
can add heparinase to a DNA solution to remove heparin, and you can heat
treat a DNA solution to remove EDTA. Has anybody tried these tricks?
Do they really work?
John Curtin School of Medical Research
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