Optimum conditions for Mitochondrial PCR
tara.paton at utoronto.ca
Tue Aug 20 11:11:52 EST 1996
Has anyone worked out optimum conditions for performing PCR on
mitochondrial DNA? I'm doing molecular evolution analysis on
mitochondrial DNA and need a large amount of product! I was told that
the concentration of Mg in the buffer was critical for this to work.
If anyone knows a good protocol/reagents for this kind of PCR, I would
appreciate hearing about it. Thanks.
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