Optimum conditions for Mitochondrial PCR

Tara Paton tara.paton at utoronto.ca
Tue Aug 20 11:11:52 EST 1996


Has anyone worked out optimum conditions for performing PCR on 
mitochondrial DNA?  I'm doing molecular evolution analysis on 
mitochondrial DNA and need a large amount of product!  I was told that 
the concentration of Mg in the buffer was critical for this to work.  
If anyone knows a good protocol/reagents for this kind of PCR, I would 
appreciate hearing about it.  Thanks.

Tara Paton    




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