DNA gel extraction

Paul N Hengen pnh at ncifcrf.gov
Tue Aug 20 15:35:23 EST 1996

Taek H. You (tyou at postbox.acs.ohio-state.edu) wrote:

> I frequently use a gel extraction method where I poke a hole at the
> bottom of a .5 ml tume and plug some whatman with T.E. to spin a gel
> through to extract DNA.  Does anyone know where this technique came
> from?  Original publication would be nice.
> Thanks in advance.

: I don't remember exactly, but I'm pretty sure that there was a little
: techniques article in NAR about 2 years ago that described this method.

> I thought it was from BioTechniques.
> The least I can say is that the described method or similar method was on 
> BioTechniques a while ago.

One that I know of is:

Ultrafast DNA Recovery from Agarose By Centrifugation Through a Paper Slurry
Chuang, S.-E.; Blattner, F. R.  BioTechniques 17:4:634-636

I'm pretty sure that there was one in BioTechniques much earlier 
on using a paper disk punched out of whatman paper and a tube with
a pin hole in the bottom (1987-1990), but I can't find it at their
search site.

* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - -  Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - -  *
* -  TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - -  *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/  *

More information about the Methods mailing list