In article <3218448D.5C42 at bsa.bristol.ac.uk>, "Dr Andrew J. Doherty" <doherty at bsa.bristol.ac.uk> wrote: > should, but then shouldn't the primers have 5'phosphates any way? Actually, they should not. You do need to kinase your primers or PCR products to clone into dephosph. vector. Regards, V.