Cloning PCR products

Vladimir Svetlov vvsvetlov at utmem1.utmem.edu
Mon Aug 19 20:11:59 EST 1996


In article <3218448D.5C42 at bsa.bristol.ac.uk>, "Dr Andrew J. Doherty"
<doherty at bsa.bristol.ac.uk> wrote:

> should, but then shouldn't the primers have 5'phosphates any way?

Actually, they should not. You do need to kinase your primers or PCR
products to clone into dephosph. vector. 
Regards,
V.



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